Chemical Composition and Evaluation of Anti-tyrosinase and Anti-Oxidative Effects of Topical Cream Formulation from Acacia sieberiana, Vitellaria paradoxa and Beeswax

Skin diseases can get natural therapies from medicinal plant-based products. In this study, a topical cream was formulated ethanol extract of Acacia sieberiana, beeswax and Vitellaria paradoxa (shea) butter. GC-MS characterization with co-injection the revealed Stearic acid (31.43%), Palmitic (23.15%), Oleic (21.44%) Linoleic (16.20%) as major components. Seven phenolic conpounds were identified quantified by HPLC- DAD Ferulic (12.81±0.26 mg/g) most abundant. The showed good antioxidant properties evaluated through ?-Carotene-linoleic assay, DPPH• radical scavenging, ABTS•+ CUPRAC metal chelating assay. had higher activity in assay (IC50 = 32.10±0.84 µg/mL), 22.49±0.62 µg/mL) 49.27±0.79 than ?-Tocopherol. effects are an indication that reduce oxidative stress on skin including aging, carcinogenesis inflammation. At 100 µg/mL, tyrosinase inhibition 48.23±0.87% regarded relatively compared to standard inhibitor kojic acid, which 79.50±0.32% at same concentration. cosmetic able inhibit melanin production rate-limiting enzyme, tyrosinase, indicating it control hyperpigmentation spots.